The following is from the U.S. EPA website on water quality monitoring

5.2 Dissolved Oxygen and Biochemical Oxygen Demand

What is dissolved oxygen and why is it important?

The stream system both produces and consumes oxygen. It gains oxygen from the atmosphere and from plants as a result of photosynthesis. Running water, because of its churning, dissolves more oxygen than still water, such as that in a reservoir behind a dam. Respiration by aquatic animals, decomposition, and various chemical reactions consume oxygen.

Wastewater from sewage treatment plants often contains organic materials that are decomposed by microorganisms, which use oxygen in the process. (The amount of oxygen consumed by these organisms in breaking down the waste is known as the biochemical oxygen demand or BOD. A discussion of BOD and how to monitor it is included at the end of this section.) Other sources of oxygen-consuming waste include stormwater runoff from farmland or urban streets, feedlots, and failing septic systems.

Oxygen is measured in its dissolved form as dissolved oxygen (DO). If more oxygen is consumed than is produced, dissolved oxygen levels decline and some sensitive animals may move away, weaken, or die.

DO levels fluctuate seasonally and over a 24-hour period. They vary with water temperature and altitude. Cold water holds more oxygen than warm water (Table 5.3) and water holds less oxygen at higher altitudes. Thermal discharges, such as water used to cool machinery in a manufacturing plant or a power plant, raise the temperature of water and lower its oxygen content. Aquatic animals are most vulnerable to lowered DO levels in the early morning on hot summer days when stream flows are low, water temperatures are high, and aquatic plants have not been producing oxygen since sunset.

Table 5.3

Maximum dissolved oxygen concentrates vary with temperature

0 14.60 23 8.56
1 14.19 24 8.40
2 13.81 25 8.24
3 13.44 26 8.09
4 13.09 27 7.95
5 12.75 28 7.81
6 12.43 29 7.67
7 12.12 30 7.54
8 11.83 31 7.41
9 11.55 32 7.28
10 11.27 33 7.16
11 11.01 34 7.16
12 10.76 35 6.93
13 10.52 36 6.82
14 10.29 37 6.71
15 10.07 38 6.61
16 9.85 39 6.51
17 9.65 40 6.41
18 9.45 41 6.41
19 9.26 42 6.22
20 9.07 43 6.13
21 8.90 44 6.04
22 8.72 45 5.95


Sampling and Equipment Considerations

In contrast to lakes, where DO levels are most likely to vary vertically in the water column, the DO in rivers and streams changes more horizontally along the course of the waterway. This is especially true in smaller, shallower streams. In larger, deeper rivers, some vertical stratification of dissolved oxygen might occur. The DO levels in and below riffle areas, waterfalls, or dam spillways are typically higher than those in pools and slower-moving stretches. If you wanted to measure the effect of a dam, it would be important to sample for DO behind the dam, immediately below the spillway, and upstream of the dam. Since DO levels are critical to fish, a good place to sample is in the pools that fish tend to favor or in the spawning areas they use.

An hourly time profile of DO levels at a sampling site is a valuable set of data because it shows the change in DO levels from the low point just before sunrise to the high point sometime in the midday. However, this might not be practical for a volunteer monitoring program. It is important to note the time of your DO sampling to help judge when in the daily cycle the data were collected.

DO is measured either in milligrams per liter (mg/L) or "percent saturation." Milligrams per liter is the amount of oxygen in a liter of water. Percent saturation is the amount of oxygen in a liter of water relative to the total amount of oxygen that the water can hold at that temperature.




What is biochemical oxygen demand and why is it important?

Biochemical oxygen demand, or BOD, measures the amount of oxygen consumed by microorganisms in decomposing organic matter in stream water. BOD also measures the chemical oxidation of inorganic matter (i.e., the extraction of oxygen from water via chemical reaction). A test is used to measure the amount of oxygen consumed by these organisms during a specified period of time (usually 5 days at 20 C). The rate of oxygen consumption in a stream is affected by a number of variables: temperature, pH, the presence of certain kinds of microorganisms, and the type of organic and inorganic material in the water.

BOD directly affects the amount of dissolved oxygen in rivers and streams. The greater the BOD, the more rapidly oxygen is depleted in the stream. This means less oxygen is available to higher forms of aquatic life. The consequences of high BOD are the same as those for low dissolved oxygen: aquatic organisms become stressed, suffocate, and die.

Sources of BOD include leaves and woody debris; dead plants and animals; animal manure; effluents from pulp and paper mills, wastewater treatment plants, feedlots, and food-processing plants; failing septic systems; and urban stormwater runoff.

The table below shows Dissolved Oxygen levels in various streams in the Matfield Basin. Site's MR1, MR2 and MR3 are the Matfield River, site SR1 is the Satucket. These two sites make the best comparison for Dissolved Oxygen. The other streams are very small with little or no flow in the summer time as you can see by the flow figures in the far left column.

The EPA standard for Dissolved Oxygen is a minimum of 5.0 milligrams per liter (5.0 mg/L). As you can see in the table below the Matfield does not meet this standard.

The table below is DO sampling from the Salisbury Plain River. Site SPR1 is below the Brockton Plant sites SPR2 and SPR3 are above the plant in the city. SPR1 has a mean DO of 3.3, that's pitiful.